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custom affymetrix based dna microarray  (Thermo Fisher)


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    Thermo Fisher custom affymetrix based dna microarray
    FIGURE 1. Purification of B cells and T cells from mixed splenocytes before and after activation. CD8 T cells, CD4 T cells, and B cells were purified from mixed splenocytes before or after activation using the MACS system with anti-CD8-, anti-CD4-, or anti-CD19-coated magnetic beads, respectively. Phenotypic analysis of purified fresh and activated cell populations was performed by flow cytometry and showed 90% purity of all cell preparations, as indicated. Purified cells were used either for RNA preparation for <t>microarray</t> analysis, or were processed to extract glycans for MALDI profiling and methylation analysis.
    Custom Affymetrix Based Dna Microarray, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/custom affymetrix based dna microarray/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    custom affymetrix based dna microarray - by Bioz Stars, 2026-05
    99/100 stars

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    1) Product Images from "Activation of murine CD4+ and CD8+ T lymphocytes leads to dramatic remodeling of N-linked glycans."

    Article Title: Activation of murine CD4+ and CD8+ T lymphocytes leads to dramatic remodeling of N-linked glycans.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.177.4.2431

    FIGURE 1. Purification of B cells and T cells from mixed splenocytes before and after activation. CD8 T cells, CD4 T cells, and B cells were purified from mixed splenocytes before or after activation using the MACS system with anti-CD8-, anti-CD4-, or anti-CD19-coated magnetic beads, respectively. Phenotypic analysis of purified fresh and activated cell populations was performed by flow cytometry and showed 90% purity of all cell preparations, as indicated. Purified cells were used either for RNA preparation for microarray analysis, or were processed to extract glycans for MALDI profiling and methylation analysis.
    Figure Legend Snippet: FIGURE 1. Purification of B cells and T cells from mixed splenocytes before and after activation. CD8 T cells, CD4 T cells, and B cells were purified from mixed splenocytes before or after activation using the MACS system with anti-CD8-, anti-CD4-, or anti-CD19-coated magnetic beads, respectively. Phenotypic analysis of purified fresh and activated cell populations was performed by flow cytometry and showed 90% purity of all cell preparations, as indicated. Purified cells were used either for RNA preparation for microarray analysis, or were processed to extract glycans for MALDI profiling and methylation analysis.

    Techniques Used: Activation Assay, Magnetic Beads, Cytometry, Microarray, Methylation



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    custom affymetrix based dna microarray  (Thermo Fisher)
    99
    Thermo Fisher custom affymetrix based dna microarray
    FIGURE 1. Purification of B cells and T cells from mixed splenocytes before and after activation. CD8 T cells, CD4 T cells, and B cells were purified from mixed splenocytes before or after activation using the MACS system with anti-CD8-, anti-CD4-, or anti-CD19-coated magnetic beads, respectively. Phenotypic analysis of purified fresh and activated cell populations was performed by flow cytometry and showed 90% purity of all cell preparations, as indicated. Purified cells were used either for RNA preparation for <t>microarray</t> analysis, or were processed to extract glycans for MALDI profiling and methylation analysis.
    Custom Affymetrix Based Dna Microarray, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/custom affymetrix based dna microarray/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    custom affymetrix based dna microarray - by Bioz Stars, 2026-05
    99/100 stars
    		  Buy from Supplier

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    FIGURE 1. Purification of B cells and T cells from mixed splenocytes before and after activation. CD8 T cells, CD4 T cells, and B cells were purified from mixed splenocytes before or after activation using the MACS system with anti-CD8-, anti-CD4-, or anti-CD19-coated magnetic beads, respectively. Phenotypic analysis of purified fresh and activated cell populations was performed by flow cytometry and showed 90% purity of all cell preparations, as indicated. Purified cells were used either for RNA preparation for microarray analysis, or were processed to extract glycans for MALDI profiling and methylation analysis.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Activation of murine CD4+ and CD8+ T lymphocytes leads to dramatic remodeling of N-linked glycans.

    doi: 10.4049/jimmunol.177.4.2431

    Figure Lengend Snippet: FIGURE 1. Purification of B cells and T cells from mixed splenocytes before and after activation. CD8 T cells, CD4 T cells, and B cells were purified from mixed splenocytes before or after activation using the MACS system with anti-CD8-, anti-CD4-, or anti-CD19-coated magnetic beads, respectively. Phenotypic analysis of purified fresh and activated cell populations was performed by flow cytometry and showed 90% purity of all cell preparations, as indicated. Purified cells were used either for RNA preparation for microarray analysis, or were processed to extract glycans for MALDI profiling and methylation analysis.

    Article Snippet: 6 The online version of this article contains supplemental material. at B row n U niversity on M ay 30, 2014 http://w w w .jim m unol.org/ D ow nloaded from Microarray analysis of the expression of cytokine and glycan transferase genes For gene expression analysis, mRNAs were extracted from purified cell populations of fresh and activated lymphocytes and subjected to analysis on a custom Affymetrix-based DNA microarray containing murine and human glycosyltransferase, sulfotransferase, and cytokine genes, made available by the Consortium for Functional Glycomics.

    Techniques: Activation Assay, Magnetic Beads, Cytometry, Microarray, Methylation